bioinformatics

Genomic and Molecular Services

whole genome & exome sequencing

MOgene offers whole genome and exome sequencing services for short and long read technology, and supports data analysis needs from Illumina and PacBio platforms.

Illumina Short Read Sequencing

de novo sequencing and generation of assemblies targeting microorganisms and polishing long read assembly
Genotyping and structural variant analyses
Genome annotation and gene prediction
Genome resequencing
Metagenomic analyses

PacBio SMRT Sequencing

de novo sequencing and generation of assemblies for complete microorganisms and eukaryotes
Structural variant analyses
Genome resequencing

TARGETED SEQUENCING, GENETIC MODIFICATIONS AND GENOTYPING

MOgene offers confidence, experience, and flexibility in deciding the optimal platform for evaluating genetic modifications and genotypes, in addition to short and long read targeted sequencing and associated analysis requirements.

Illumina Short Read Sequencing

Targeted capture for genotyping SNPs, CNVs, and indels
Comparisons of genes of interest in different cell types
Metagenomic analysis
Amplicon sequencing

PacBio SMRT Sequencing

Transgene integration structure to determine stability in recombinant cell lines
Characterization of novel cell lines used in biological production processes
Comparison of genes of interest in different cell populations
Identification of alleles for the genes of interest
Amplicon sequencing

transcriptomics

MOgene offers confidence, experience, and flexibility with your transcriptomics requirements and can process differential gene expression data, identify splice variants, and conduct absolute or relative quantification of gene expression.

Next Generation Sequencing Applications

RNA-Seq (Illumina) differential gene expression
Iso-Seq transcriptome generation (PacBio)
Small RNA sequencing (Illumina)

Agilent Microarrays

As a Certified Agilent Services Provider since 2004, MOgene offers the number one solution for your microarray needs.

Differential gene expression
Differential microRNA expression

INFORMATICS TOOLS AND DATA DELIVERY

MOgene offers customization of pipelines to meet data analysis requirements and various options for secure data delivery.

Informatics Tools

PacBio Tools

Subread manipulation and demultiplexing
Assembly
IsoSeq transcriptome analysis
Alignment and resequencing variant detection

Illumina Tools

Assembly, alignment, and variant detection (GATK)
Read adapter and quality trimmer
FastQC quality viewer
Flash paired read merging tool

Recognized Bioinformatics Tools

QIIME2 metagenomics
Blast suite, R, Bioconductor, BioPython, samtools, vcftools, Perl
BWA alignment
Tuxedo suite for reference guided RNAseq (Bowtie)
Trinity suite for de novo RNAseq
Tools to find open reading frames in prokaryotes and eukaryotes
Mauve tool to graphically display the relationships between related assemblies
Assemblers including: CLC (Qiagen), Spades, Velvet, Mira
Genome viewers: IGV, Tablet

Data Delivery

Amazon’s AWS S3 secure storage
SendThisFile temporary link for data up to ~10GB
Portable hard disk drive (HDD)
USB stick
Dropbox
Google Drive

Technology Platforms

Quality and safety

The Quality Commitment From MOgene

Regulatory Services

MOgene has a state-of-the-art, CLIA certified facility with an established reputation of complying with relevant national and international GxP compliance regulations.

Audit ready for Clients with routine internal quality audits
GLP compliance per FDA 21 CFR Part 58 and Part 11
Active CAPA program for process improvement
CLIA certification
USDA certification

Quality Management System (QMS)

MOgene has a robust Quality Management System (QMS) detailed in a manual that ensures compliance for quality monitoring and improvement.

Rigorous sample management process
Transparent and routine communications on project timelines and deliverables
Complete documentation of standard operating procedures (SOPs) and 100% QC of data

BioSafety

Our Biosafety practices enable us to provide services for early R&D and market release studies for pathogens that require BLS-2+ facilities.

Higher levels of lab safety for antimicrobial, human and animal health vaccine development
Dedicated BSL-2+ lab and pre-amplification room for your clinical studies
Trained Personnel to handle all kinds of BSL-2+ level agents
UNIDIRECTIONAL workflow