PACBIO sequencing

Genomic and Molecular Services

PACBIO SEQUENCING

MOgene now offers all sequencing services on the PacBio Sequel sequencing system. The Sequel system, a next generation of SMRT (Single-molecule, real-time) sequencing system, offers much higher throughput and more scalability than the Second-Generation Sequencing (SGS) technologies, making it well-suited for unsolved problems in genome, transcriptome, and epigenetics research.

The highly-contiguous de novo assemblies using PacBio sequencing can close gaps in current reference assemblies and characterize structural variation (SV) in personal genomes. With longer reads, we can sequence through extended repetitive regions and detect mutations, many of which are associated with diseases. Moreover, PacBio transcriptome sequencing is advantageous for the identification of gene isoforms and facilitates reliable discoveries of novel genes and novel isoforms of annotated genes, due to its ability to sequence full-length transcripts or fragments with significant lengths.

Additionally, PacBio’s sequencing technique provides information that is useful for the direct detection of base modifications, such as methylation. In addition to using PacBio sequencing alone, many hybrid sequencing strategies, such as combination of PacBio sequencing and Illumina data, have been developed to make use of more accurate short reads in conjunction with PacBio long reads. In general, hybrid sequencing strategies are more affordable and scalable especially for small-size laboratories than using PacBio Sequencing alone. The advent of PacBio sequencing has made available much information that could not be obtained via SGS alone.

Applications include

de novo Assembly
Exomes and whole genomes
Targeted Sequencing
Base modifications
Isoform sequencing detection
Amplicon\COSMID sequencing

Areas include

Human
Plant & Animal
Microbiology

PacBio Sample Submission Guidelines

Please review following documents for DNA or RNA (IsoSeq) sample submission and shipping guidelines.

Quality and safety

The Quality Commitment From MOgene

Regulatory Services

MOgene has an established reputation of complying with relevant national and international quality compliance regulations.

Audit ready for clients with routine internal quality audits
GLP compliance per FDA 21 CFR Part 58 and Part 11
Active CAPA program for process improvement
CLIA Certification
USDA Certification

Quality Management System

MOgene has a robust Quality Management System (QMS) detailed in a manual that ensures compliance for quality monitoring and improvement.

Transparent and routine communications on project timelines and deliverables
Complete documentation of standard operating procedures (SOPs) and 100% QC of data
Rigorous sample management process

BioSafety

Our Biosafety practices enable us to provide services for early R&D and market release studies for pathogens that require BSL-2+ facilities.

Higher levels of lab safety for antimicrobial, human and animal health vaccine development
Dedicated BSL-2+ Lab and pre-amplification room for your clinical studies
Trained personnel to handle BSL-2+ level agents
UNIDIRECTIONAL workflow