by Bill Curtiss | May 13, 2021 | Blog
Instead of the relative quantification of real-time polymerase chain reaction (qPCR), droplet digital PCR (ddPCR) ensures absolute quantification – an effortless determination of the number of target sequences in the original sample without the use of standard...
by Bill Curtiss | May 6, 2021 | Blog
Digital droplet polymerase chain reaction (ddPCR) is a relatively new form of PCR with a wide range of applications in clinical, animal, plant, and environmental studies. ddPCR is a variant of digital PCR based on a water-oil emulsion system. Its main principle is...
by Bill Curtiss | Apr 29, 2021 | Blog
In 1971, a paper was published in the Journal of Molecular Biology that first described the process of using heat-resistant enzymes together with primers to replicate a short DNA sequence in vitro. The actual invention of the polymerase chain reaction (PCR) came over...
by Bill Curtiss | Apr 22, 2021 | Blog
Polymerase chain reaction (PCR) was first developed in 1983 by an American biochemist named Kary Mullis. This biochemical method aims to amplify small DNA samples from biological source materials and produce millions to billions of copies of that specific DNA...